A methodological approach to simultaneous quantification of glycerol and fatty acids from cork suberin

In the present work an alternative methanolysis work-up methodology was developed for the isolation of depolymerized suberin in order to quantify accurately all suberin building blocks, including glycerol, in the same GC run. GC-FID response factors were determined for glycerol and each suberin fatty acid substructure with internal standards. Glycerol represented 38.4 molar% of suberin and COOH/OH groups ratio was 0.6:1 in contrast with 0.10 area% and COOH/OH ratio of 3:1 in the most used methanolysis methodology

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