1. Biodiversity and conservation: joint effects of landscape connectivity and global changes on species, communities and biodiversity; 2. Macroecology: empirical modelling of species distribution and biodiversity; 3. Effects of multiple stressors on freshwater biota; 4. Biotic integrity of river ecosystems; 5. Herpetology with emphasis on the ecology and conservation of freshwater turtles.


No news found.


Scientific Articles (ISI)

Marcolin F., Segurado P., Chamberlain D., Reino L. (2023)

Testing the links between bird diversity, alien species and disturbance within a human-modified landscape.

Ecography, e06886. DOI:

Duarte G., Branco P., Haidvogl G., Ferreira M.T., Pont D., Segurado P. (2022)

iPODfish – A new method to infer the historical occurrence of diadromous fish species along river networks

Science of the Total Environment, 812: 152437. DOI:

Segurado P., Gutiérrez-Cánovas C., Ferreira M.T., Branco P. (2022)

Stressor gradient coverage affects interaction identification

Ecological Modelling, 472: 110089. DOI:

Almeida C., Branco P., Segurado P., Ramos T.B., Ferreira M.T., Neves R., de Oliveira R.P. (2021)

Evaluation of the trophic status in a Mediterranean reservoir under climate change: an integrated modelling approach

Journal of Water and Climate Change, 12(3): 817-832. DOI:

Branco P., Segurado P., Costa M.J., Teixeira A., Santos J.M., Ferreira M.T., Duarte G. (2021)

Knowledge gaps in the definition of threats for the red list assessment of European freshwater-dependent fish species

Biology, 10(7): 680. DOI:

Costa M.J., Duarte G., Segurado P., Branco P. (2021)

Major threats to European freshwater fish species

Science of the Total Environment, 797: 149105. DOI:

Duarte G., Segurado P., Haidvogl G., Pont D., Ferreira M.T., Branco P. (2021)

Damn those damn dams: fluvial longitudinal connectivity impairment for European diadromous fish throughout the 20th century

Science of the Total Environment, 761: 143293. DOI:

Feio M.J., Hughes R.M., Callisto M., Nichols S.J., Odume O.N., Quintella B.R.g, Kuemmerlen M., Aguiar F.C., Almeida S.F.P., Alonso-Eguíalis P., Arimoro F.O., Dyer F.J., Harding J.S., Jang S., Kaufmann P.R., Lee S., Li J., Macedo D.R., Mendes A., Mercado-Silva N., Monk W., Nakamura K., Ndiritu G.G., Ogden R., Peat M., Reynoldson T.B., Rios-Touma B., Segurado P., Yates A.G. (2021)

The biological assessment and rehabilitation of theworld’s rivers: an overview

Water (Switzerland), 13 (3): 371. DOI:

Loulida S., Segurado P., Naimi M., Znari M. (2021)

Niche differentiation among genetic lineages in the Mediterranean Pond turtle, Mauremys leprosa, across its geographical range

Amphibia-Reptilia, 42(2), 227-240. DOI:

Segurado P., Ferreira M.T., Branco P. (2021)

Assessing the effects of multiple stressors on aquatic systems across temporal and spatial scales: from measurement to management

Water, 13(24): 3549. DOI:

Spears B.M., Chapman D.S., Carvalho L., Feld C. K., Gessner M.O., Piggott J.J., Lindsay F.Banin Gutiérrez-Cánovas C., Solheim A.L., Richardson J.A., Schinegger R., Segurado P., Thackeray S. J., Birk S. (2021)

Making waves. Bridging theory and practice towards multiple stressor management in freshwater ecosystems

Water Research, 196: 116981. DOI:

Vilizzi L., …, Branco P., …, Ferreira M.T., …, Santos J.M., …, Segurado P. et al. (2021)

A global-scale screening of non-native aquatic organisms to identify potentially invasive species under current and future climate conditions

Science of the Total Environment, 788: 147868. DOI:

Carreira B.M., Segurado P., Laurila A., Rebelo R. (2020)

Heat waves trigger swift changes in the diet and life-history of a freshwater snail

Hydrobiologia, 847(4): 999-1011. DOI:

Molina-Navarro E., Segurado P., Branco P., Almeida C., Andersen H.E. (2020)

Predicting the ecological status of rivers and streams under different climatic and socioeconomic scenarios using Bayesian Belief Networks

Limnologica, 80: 125742. DOI:

Serrano F., Pita R., Mota-Ferreira M., Beja P., Segurado P. (2020)

Landscape connectivity affects individual survival in unstable patch networks: the case of a freshwater turtle inhabiting temporary ponds

Freshwater Biology, 65(3): 540-551. DOI:

Duarte G., Segurado P., Oliveira T., Haidvogl G., Pont D., Ferreira M.T., Branco P. (2019)

The River Network Toolkit ‚Äď RivTool.

Ecography, 42:1-9. DOI:

Godinho F.N., Segurado P., Franco A., Pinheiro P., P√°dua J., Rivaes R., Ramos P. (2019)

Factors related to fish kill events in Mediterranean reservoirs.

Water Research, 158: 280-290. DOI:

Oliveira J.M., Paulino J.C., Segurado P., Rosado J., Rivaes R., Fernandes F., Serafim P., Ferrreira M.T. (2019)

Aquatic biodiversity and quality of streams in certified eucalypt plantations in Central Portugal

Forest Sciences, 47(123): 383-394.

Ros√°rio I.T., Rebelo R., Cardoso P., Segurado P., Mendes R.N., Santos-Reis M. (2019)

Can geocaching be an indicator of cultural ecosystem services? The case of the montado savannah-like landscape

Ecological Indicators, 99: 375-386.

Aguiar F.C., Segurado P., Martins M.J., Bejarano M.D., Nilsson C., Portela M.M., Merritt D. (2018)

The abundance and distribution of guilds of riparian woody plants change in response to land use and flow regulation.

Journal of Applied Ecology, 55(5): 2227-2240. DOI:

Almeida C., Ramos T.B., Segurado P., Branco P., Neves R., de Oliveira R.P. (2018)

Water quantity and quality under future climate and societal scenarios: a basin-wide approach applied to the Sorraia River, Portugal.

Water, 10(9):1186.

Duarte G., Moreira M., Branco P., Costa L., Ferreira M.T., Segurado P. (2018)

One millennium of historical freshwater fish occurrence data for Portuguese rivers and streams.

Scientific Data, 5: 180163. DOI:

Hering D., Borja A., Jones J.I., Pont D., Boets P., Bouchez A., Bruce K., Drakare S., Hänfling B., Kahlert M., Leese F., Meissner K., Mergen P., Reyjol Y., Segurado P., Vogler A., Kelly M. (2018)

Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive.

Water Research, 138: 192-205.

Hermoso V., Filipe A.F., Segurado P., Beja P. (2018)

Freshwater conservation in a fragmented world: dealing with barriers in a systematic planning framework.

Aquatic Conservation: Marine and Freshwater Ecosystems, 28(1): 17-25.

Lozanovska I., Ferreira M.T., Segurado P., Aguiar F.C. (2018)

Limited resilience in hotspots of functional richness: the Mediterranean riparian shrublands.

Aquatic Sciences, 80: 25.

Reino L., Trivi√Īo M., Beja P., Ara√ļjo M.B., Figueira R., Segurado P. (2018)

Modelling landscape constraints on farmland bird species range shifts under climate change.

Science of the Total Environment, 625: 1596‚Äď1605.

Segurado P., Almeida C., Neves R., Ferreira M.T., Branco P. (2018)

Understanding multiple stressors in a Mediterranean basin: combined effects of land use, water scarcity and nutrient enrichment.

Science of the Total Environment, 624: 1221-1233.

Carreira B.M., Segurado P., Laurila A., Rebelo R. (2017)

Can heat waves change the trophic role of the world’s most invasive crayfish? Diet shifts in Procambarus clarkii.

PLoS ONE, 12(9): e0183108.

Reino L., Ferreira M., Martínez-Solano I., Segurado P., Xu C., Barbosa A.M. (2017)

Favourable areas for co- occurrence of parapatric species: niche conservatism and niche divergence in Iberian tree frogs and midwife toads.

Journal of Biogeography, 44(1): 88‚Äď98. DOI:

Teichert N., Lepage M., Sagouis A., Borja A., Chust G., Ferreira M.T., Pasquaud S., Schinegger R., Segurado P., Argillier C. (2017)

Functional redundancy and sensitivity of fish assemblages in European rivers, lakes and estuarine ecosystems.

Scientific Reports, 7: 17611.

Carreira B.M., Segurado P., Orizaola G., Gonçalves N., Pinto V., Laurila A., Rebelo R. (2016)

Warm vegetarians? Heat waves and diet shifts in tadpoles.

Ecology, 97(11): 2964‚Äď2974.

Feld C.K., Segurado P., Gutiérrez-Cánovas C. (2016)

Analysing the impact of multiple stressors in aquatic biomonitoring data: a ‚Äúcookbook‚ÄĚ with applications in R.

Science of the Total Environment,573: 1320‚Äď1339.

Fernandes M.R., Segurado P., Jauch E., Ferreira M.T. (2016)

Riparian responses to extreme climate and land-use change scenarios.

Science of the Total Environment, 569-570: 145-158.

Hermoso V., Filipe A.F., Segurado P., Beja P. (2016)

Catchment zoning to unlock freshwater conservation opportunities in the Iberian Peninsula.

Diversity and Distributions, 22 (9): 960‚Äď969.

Schinegger R., Palt M., Segurado P., Schmutz S. (2016)

Untangling the effects of multiple human stressors and their impacts on fish assemblages in European running waters.

Science of the Total Environment, 573:1079-1088.

Segurado P., Branco P., Jauch E., Neves R., Ferreira M.T. (2016)

Sensitivity of river fishes to climate change: the role of hydrological stressors on habitat range shifts.

Science of the Total Environment, 562: 435-445.

Hermoso V., Filipe A.F., Segurado P., Beja P. (2015)

Filling gaps in a large reserve network to address freshwater conservation needs.

Journal of Environmental Management, 161: 358-365.

Hermoso V., Filipe A.F., Segurado P., Beja P. (2015)

Effectiveness of a large reserve network in protecting freshwater biodiversity: a test for the Iberian Peninsula.

Freshwater Biology, 60: 698-710.

Moreira F.D., Ascens√£o F., Capinha C., Rodrigues D., Segurado P., Santos-Reis M., Rebelo R. (2015)

Modelling the risk of invasion by the red-swamp crayfish (): incorporating local variables to better inform management decisions.

Biological Invasions, 17: 273‚Äď285.

Segurado P., Branco P., Avelar A.P., Ferreira M.T. (2015)

Historical changes in the functional connectivity of rivers based on spatial network analysis and the past occurrences of diadromous species in Portugal.

Aquatic Sciences, 77(3): 427-440.

Aguiar F.C., Segurado P., Urbanic G., Cambra J., Chauvin C., Ciadamidaro S,. Dörflinger G., Ferreira J., Germ M., Manolaki P., Minciardi M.R., Munné A., Papastergiadou E., Ferreira M.T. (2014)

Comparability of river quality assessment using macrophytes: a multi-step procedure to overcome biogeographical differences.

Science of the Total Environment, 476-477: 757-767. DOI:

Branco P., Segurado P., Santos J.M., Ferreira M.T. (2014)

Prioritizing barrier removal to improve functional connectivity of rivers.

Journal of Applied Ecology, 51(5): 1197-1206.

Segurado P., Caiola N., Pont D., Oliveira J., Delaigue O., Ferreira M.T. (2014)

Comparability of fish-based ecological quality assessments for geographically distinct Iberian regions.

Science of the Total Environment, 476-477: 785-794.

Reino L., Beja P., Ara√ļjo M.B., Dray S., Segurado P. (2013)

Does local habitat fragmentation affect large-scale distributions? The case of a specialist grassland bird.

Diversity and Distributions, 19(4): 423-432.

Segurado P., Branco P., Ferreira M.T. (2013)

Prioritizing restoration of structural connectivity in rivers: a graph based approach.

Landscape Ecology, 28(7): 1231-1238.

Branco P.J., Segurado P., Santos J.M., Pinheiro P.J., Ferreira M.T. (2012)

Does longitudinal connectivity loss affect the distribution of freshwater fish?

Ecological Engineering,48: 70-78.

Oliveira J.M.,Segurado P., Santos J.M., Teixeira A., Ferreira M.T., Cortes R.V. (2012)

Modelling stream-fish functional traits in reference conditions: Regional and local environmental correlates.

PLoS ONE, 7(9): e45787.

Segurado P., Kunin W.E., Filipe, A.F., Ara√ļjo M.B. (2012)

Patterns of coexistence of two freshwater turtles are affected by spatial scale.

Basic and Applied Ecology, 13: 371-379.

Pereira M., Segurado P., Neves, N. (2011)

Using spatial network structure in landscape management and planning: a case study with pond turtles.

Landscape and Urban Planning, 100(1-2): 67-76.

Segurado P., Santos J.M., Pont D., Melcher A., Jalon D.G., Hughes R.M., Ferreira M.T. (2011)

Estimating species tolerance to human perturbation: expert judgment versus empirical approaches.

Ecological Indicators, 11(6): 1623-1635.

Godinho C., Rabaça C.J., Segurado P. (2010)

Breeding bird assemblages in riparian galleries of the Guadiana river basin (Portugal): the effect of spatial structure and habitat variables.

Ecological Research, 25: 283-294.

Aguiar F.C., Ferreira M.T., Albuquerque A., Rodríguez-González P.M., Segurado P. (2009)

Structural and functional responses of riparian vegetation to human disturbance: performance and spatial-scale dependence.

Fundamental and Applied Limnology, 175(3): 249-267.


Assessing the Effects of Multiple Stressors on Aquatic Systems across Temporal and Spatial Scales: From Measurement to Management

Segurado, P.; Ferreira, M.T.; Branco, P. (Eds) (2022)

MDPI, Basel, Switzerland. ISBN: 978-3-0365-4199-0 DOI:

Book Chapters

Indjai B., Segurado P., Romeiras M.M., Catarino L. (2023)

Bioecologia e import√Ęncia socioecon√≥mica das palmeiras na √Āfrica Ocidental

In: Catarino, S., Fortes, A., Ferreira, V., Duarte, M.C (eds). Flora and Natural Resources of Cabo Verde Islands. Universidade de Cabo Verde e Universidade de Lisboa. ISAPress, Lisboa, 38p.. ISBN: 978-989-35095-2-4 URL:

Rebelo R., Carretero M., Gonçalves H., Segurado P., Sequeira F., Lopes I. (2019)

Capítulo 9. Anfíbios e répteis.

In: Rios de Portugal: comunidades, processos e altera√ß√Ķes (Coord. Feio M.J., Ferreira V.), p. 203-231. Imprensa da Universidade de Coimbra, Coimbra. ISBN: 978-989-26-1623-0 DOI:

Ribeiro F., Aguiar C., Anastácio P., Capinha C., Grosso-Silva J.M., Matias R.,, Polónia A., Segurado P., Sousa A., Sousa R., Alves P. (2018)

Breve Hist√≥ria das Introdu√ß√Ķes Biol√≥gicas em Portugal Continental

In: Breve Hist√≥ria das Introdu√ß√Ķes Biol√≥gicas em Portugal Continental. In: As Invas√Ķes Biol√≥gicas em Portugal: Hist√≥ria, Diversidade e Gest√£o (Coord. Vaz A.S., Eds. Vicente J.R., Queiroz A.I., Marchante E., Honrado J.P., Silva L), p. 62-84. Arte e Ci√™ncia, Porto. ISBN: 978-989-99518-8-4

Aguiar F.C., Martins M.J., Bejarano M.D., Nilsson C., Portela M.P., Segurado P., Merritt D.M. (2014)

Are dams regulating diversity of riparian forests? Functional trade-offs and synergies in Mediterranean Europe

In: Biodiversity and vegetation: patterns, processes, conservation. Mucina L., Price J.N., Kalwij J.M. (Eds.). Kwongan Foundation, Perth, Australia, p.63. ISBN: 978-0-9584766-5-2

Ara√ļjo P.R., Segurado P. (2008)

Mauremys leprosa

In: Atlas dos anfíbios e répteis de Portugal. Loureiro A., Ferrand de Almeida N., Carretero M.A., Paulo O.S. (Eds.). Instituto de Conservação da Natureza e da Biodiversidade, Lisboa, pp. 132-133. ISBN: 9789896800055 URL:

Segurado P., Ara√ļjo P.R. (2008)

Emys orbicularis

In: Atlas dos anfíbios e répteis de Portugal. Loureiro A., Ferrand de Almeida N., Carretero M.A., Paulo O.S. (Eds.). Instituto de Conservação da Natureza e da Biodiversidade, Lisboa, pp. 130-131. ISBN: 9789896800055 URL:


Dammed Fish

Impacto da perda de conectividade estrutural e funcional de redes hidrogr√°ficas na biodiversidade pisc√≠cola ‚Äď Otimizando solu√ß√Ķes de gest√£o

Duration: 2022-2024

CEF Budget: 244.000 ‚ā¨


Something in the way they move: how global patterns of wildlife trade influence global invasion success

Duration: 2018-2021

CEF Budget: 35.807 ‚ā¨


Screening Alnus Glutinosa resistance to an emerging disease under climate stressors: predecting alder forests resilience across river networks.

Duration: 2018-2021

CEF Budget: 207.948 ‚ā¨


RIVEAL: Valores e serviços dos ecossistemas fluviais e das florestas ripárias em paisagens fluviais alteradas e futuros climáticos incertos

Duration: 2018-2022

CEF Budget: 180979,88 ‚ā¨


Optimal Greening of Irrigated farmland to achieve a prime environment

Duration: 2016-2021

CEF Budget: 191.226 ‚ā¨


Duero (Douro) River basin: Water resources, Water accounts and Target sustainability

Duration: 2014-2015

CEF Budget: 39.134 ‚ā¨


Managing Aquatic ecosystems and water Resources under multiple Stress

Duration: 2014-2018

CEF Budget: 428.200 ‚ā¨